Pull-down method to access the cell surface receptor for Toxoplasma gondii. Issue 5 (October 2016)

• Record Type: Journal Article
• Authors: Gong, Haiyan; Kobayashi, Kyousuke; Sugi, Tatsuki; Takemae, Hitoshi; Horimoto, Taisuke; Xuan, Xuenan; Akashi, Hiroomi; Kato, Kentaro
• Is Part Of: Parasitology international. Volume 65:Issue 5:Part B(2016:Oct.)
• Page Start: 514
• Page End: 515
• Publication Date: 2016-10
• Subjects: Pull-down method -- Mass spectrometry (MS) -- Two-dimensional gel electrophoresis -- Biotin -- Streptavidin; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2016.08.013
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: Protein X, which is expressed on the surface of Toxoplasma and is thought to interact with host molecules, was expressed as a GST recombinant protein, conjugated to sepharose 4B, and used to pull down biotin-labeled 293T cells. The product was analysis by 2D-PAGE and Western blotting. Mass spectrometry revealed the reacted spots from the gel to be heat shock proteins. Graphical abstract: Highlights: We used a pull-down assay to identify the Toxoplasma gondii cell surface receptor. This assay coupled with mass spectrometry affords access to host cell receptors. We identified heat shock proteins as receptor candidates by using this method.