A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish. Issue 18 (29th June 2016)

• Record Type: Journal Article
• Authors: Hu, Wei; Zeng, Lingyu; Wang, Yanying; Liu, Zhihong; Ye, Xiaoxue; Li, Chunya
• Is Part Of: Analyst. Volume 141:Issue 18(2016)
• Page Start: 5450
• Page End: 5455
• Publication Date: 2016-06-29
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c6an00905k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Using 6-hydroxyl-quinoline-2-benzothiazole as a two-photon fluorophore and tert -butyldiphenylsilyl as a recognition domain, a ratiometric two-photon fluorescent probe was developed for fluoride ion (F − ) determination. Two-photon microscopic imaging of F − in living HeLa Cells and zebrafish was also achieved. Abstract : Using 6-hydroxyl-quinoline-2-benzothiazole (HQB ) as a two-photon fluorophore and tert -butyldiphenylsilyl as a recognition domain for F −, a ratiometric two-photon fluorescent fluoride probe, QF, was synthesized and fully characterized.QF displays both one- and two-photon ratiometric responses towards fluoride ions in aqueous solution.QF was enabled to detect exogenous fluoride ions in living cells by a ratiometric method. Two-photon microscopic imaging of fluoride ions in living HeLa cells and zebrafish has also been achieved.QF has been demonstrated to be an excellent fluorescent probe with high selectivity, low cytotoxicity and good photostability.