Preparation, characterization, and cytotoxic effects of liposomal nanoparticles containing cisplatin: an in vitro study. (17th August 2016)

• Record Type: Journal Article
• Authors: Poy, Donya; Akbarzadeh, Azim; Ebrahimi Shahmabadi, Hasan; Ebrahimifar, Meysam; Farhangi, Ali; Farahnak Zarabi, Maryam; Akbari, Azam; Saffari, Zahra; Siami, Fatemeh
• Is Part Of: Chemical biology & drug design. Volume 88:Number 4(2016)
• Page Start: 568
• Page End: 573
• Publication Date: 2016-08-17
• Subjects: atomic force microscopy -- cisplatin -- cytotoxicity -- drug delivery -- pegylated liposome; Drugs -- Design -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; 615.19005
• DOI: 10.1111/cbdd.12786
• Languages: English
• ISSNs: 1747-0277

Abstract

Abstract : Cisplatin is a chemotherapeutic agent used for treating various malignancies. The study aimed to prepare pegylated liposomal cisplatin and evaluate its efficacy against human breast cancer cell line MCF‐7. Drug‐loaded nanoparticles were synthesized by reverse phase evaporation technique. The study is highlighted by extensive characterization of nanoparticles in terms of nanoparticle morphology, type of drug entrapment, cisplatin retention capability, and cytotoxicity effects. The size, size distribution, and zeta potential of nanodrug were estimated 142 nm, 0.33, and −22 mV, respectively. Drug‐loading efficiency was equal to 48% that occurred physically. Furthermore, high retention capability (39% of drug was released after 72 h) with significantly enhanced cytotoxicity of nanodrug (1.75 times more than the standard drug) confirmed the potency of liposomal nanoparticles as proper cisplatin carrier. Abstract : Cisplatin was encapsulated into pegylated liposomal nanoparticles by reverse phase evaporation technique. Nanoparticles were characterized by dynamic light scattering technique, atomic force microscopy (AFM), spectrophotometry, Fourier transform infrared spectroscopy, and MTT assay. Proper drug‐loading efficiency and drug retention capability, along with augment cytotoxicity effects, were observed. In addition, the results of AFM microscopy confirmed the nanoparticles formation.