Inhibition of T‐cell activation attenuates hypertension, TNFα, IL‐17, and blood–brain barrier permeability in pregnant rats with angiogenic imbalance. (1st August 2016)

• Record Type: Journal Article
• Authors: Bean, Cynthia; Spencer, Shauna‐Kay; Bowles, Teylor; Kyle, Patrick B.; Williams, Jan M.; Gibbens, Jacob; Wallace, Kedra
• Is Part Of: American journal of reproductive immunology. Volume 76:Number 4(2016)
• Page Start: 272
• Page End: 279
• Publication Date: 2016-08-01
• Subjects: blood–brain barrier -- CD4+ T cells -- HELLP syndrome -- hypertension, pregnancy brain -- TNFa; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12547
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract : Problem: Angiogenic imbalance during pregnancy is associated with immune activation, hypertension, increased T cell infiltration, and neurological insults. Method of Study: On gestational day (GD) 12, timed‐pregnant rats were infused with anti‐angiogenic factors sFlt‐1 and sEndoglin (4.7 and 7 μg/kg) to create HELLP syndrome via mini‐osmotic pumps for 8 days, with a subset of these rats having Orencia (2 mg/kg) infused on GD13. On GD19, blood–brain barrier (BBB) permeability was evaluated via Evan's Blue infusion, blood was collected for T‐cell measurements, inflammatory cytokine secretion. Brain tissues were also collected to examine inflammatory cytokine infiltration. Results: T‐cell attenuation with Orencia decreased circulating CD4 + and CD8 + T cells, circulating tumor necrosis factor alpha (TNFα) and IL‐17, BBB permeability and significantly decreased biochemical evidence of HELLP compared to untreated HELLP rats. Conclusions: These data support the hypothesis that T cells have a critical role in contributing to the pathophysiology that is seen in angiogenic imbalance during pregnancy.