A biocompatible serine functionalized nanostructured zirconia based biosensing platform for non-invasive oral cancer detection. Issue 80 (15th August 2016)

• Record Type: Journal Article
• Authors: Kumar, Suveen; Sharma, Jai Gopal; Maji, Sagar; Malhotra, Bansi Dhar
• Is Part Of: RSC advances. Volume 6:Issue 80(2016)
• Page Start: 77037
• Page End: 77046
• Publication Date: 2016-08-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra07392a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Schematic of captured antigen onto BSA/anti-CYFRA-21-1/serine/nZrO2 /ITO immunoelectrode and its electrochemical response. Abstract : We report results of studies relating to the fabrication of nontoxic, biocompatible serine functionalized nanostructured zirconia (serine/nZrO2 ) for oral cancer detection. The hydrothermally synthesized ZrO2 nanoparticles (∼4.5 nm) have been characterized using X-ray diffraction (XRD) and transmission electron microscopy (TEM). The cytotoxicity studies of serine/nZrO2 carried out using MTT assay on the HEK 293 human cell line reveal biocompatibility of the serine/nZrO2 . The serine functionalized nZrO2 are electrophoretically deposited onto a hydrolysed indium tin oxide (ITO) coated glass electrode (serine/nZrO2 /ITO). Furthermore, monoclonal antibodies (anti-CYFRA-21-1) were covalently immobilized onto the serine/nZrO2 /ITO electrode and BSA was been used for blocking of the non-specific binding sites. The fabricated BSA/anti-CYFRA-21-1/serine/nZrO2 /ITO immunoelectrode was used for label free, non-invasive and efficient detection of an oral cancer biomarker (CYFRA-21-1). The electrochemical response studies of this immunoelectrode exihibited a wider linear detection range (0.01–29 ng mL −1 ) with a remarkable detection limit (0.01 ng mL −1 ) and a quick response time (6 min) for detection of the CYFRA-21-1 antigen. The results of validation studies conducted on the fabricated immunoelectrode (BSA/anti-CYFRA-21-1/serine/nZrO2 /ITO) using saliva samples of oral cancer patients indicate a good agreement with the results obtained via enzyme linked immunosorbent assay (ELISA).