Focusing on probe-modified peptides: a quick and effective method for target identification. Issue 67 (22nd July 2016)

• Record Type: Journal Article
• Authors: Sun, Huan; Ren, Yan; Hou, Weijie; Li, Lin; Zeng, Fanqi; Li, Sisi; Ma, Yongfen; Liu, Xiao; Chen, She; Zhang, Zhiyuan
• Is Part Of: Chemical communications. Volume 52:Issue 67(2016)
• Page Start: 10225
• Page End: 10228
• Publication Date: 2016-07-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc04030f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : BTC-ABPP was developed by converting the reactants of a click conjugation from proteins (biochemistry) to peptides (chemistry) to identify the modified peptides. Abstract : A new and efficient method focusing on probe-modified peptides was developed to identify the target protein and modification site of a hit compound or a drug. This method exhibited high click conjugation efficiency and few false-positive results. The modification site further facilitated target validation, biological mechanism study and new indications exploration.