Comparison of two silica-based extraction methods for DNA isolation from bones. (September 2016)

• Record Type: Journal Article
• Authors: Rothe, Jessica; Nagy, Marion
• Is Part Of: Legal medicine. Volume 22(2016)
• Page Start: 36
• Page End: 41
• Publication Date: 2016-09
• Subjects: DNA extraction -- Bones -- Silica membrane -- Silica beads -- Sample quality; Medical jurisprudence -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Medical jurisprudence; Periodicals; 614.1
• DOI: 10.1016/j.legalmed.2016.07.008
• Languages: English
• ISSNs: 1344-6223

Abstract

Graphical abstract: Highlights: Two silica based DNA extraction methods using silica beads or silica membranes were compared. Testing of bone samples of various qualities with both methods show remarkable differences in DNA yield and purity. Bead-technology results in excellent purity but the membrane-based procedure yields significantly more DNA. The right choice of the extraction method depending on the quality of the bone sample is important. Abstract: One of the most demanding DNA extractions is from bones and teeth due to the robustness of the material and the relatively low DNA content. The greatest challenge is due to the manifold nature of the material, which is defined by various factors, including age, storage, environmental conditions, and contamination with inhibitors. However, most published protocols do not distinguish between different types or qualities of bone material, but are described as being generally applicable. Our laboratory works with two different extraction methods based on silica membranes or the use of silica beads. We compared the amplification success of the two methods from bone samples with different qualities and in the presence of inhibitors. We found that the DNA extraction using the silica membrane method results an in higher DNA yield but also in a higher risk of co-extracting impurities, which can act as inhibitors. In contrast the silica beads method shows decreased co-extraction of inhibitors but also less DNA yield. Related to our own experiences it has to be considered that each bone material should be reviewed independently regarding the analysis and extraction method. Therefore, the most ambitious task is determining the quality of the bone material, which requires substantial experience.