Molecular diversity of bacterial bamA gene involved in anaerobic degradation of aromatic hydrocarbons in mesophilic petroleum reservoirs. (October 2016)

• Record Type: Journal Article
• Authors: Ruan, Meng-Ya; Liang, Bo; Mbadinga, Serge Maurice; Zhou, Lei; Wang, Li-Ying; Liu, Jing-Feng; Gu, Ji-Dong; Mu, Bo-Zhong
• Is Part Of: International biodeterioration & biodegradation. Volume 114(2016)
• Page Start: 122
• Page End: 128
• Publication Date: 2016-10
• Subjects: bamA gene -- Anaerobic aromatic ring cleavage -- Mesophilic -- Oil reservoir; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2016.06.005
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: Aromatic hydrocarbons are usually metabolized to the central intermediate benzoyl-coenzyme A (CoA) prior to ring cleavage. 6-Oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, a key enzyme involved in the reductive benzene ring cleavage, encoded by the bam A gene can serve as a functional biomarker for detecting anaerobic degradation of aromatic compounds in different environments. In the present study, we examined the diversity the bacterial bam A gene in oil field. We sequenced bam A fragments that were PCR amplified from the microbial biomass of two mesophilic oil producing reservoirs during a period of four months. The results showed the presence and a higher diversity of bam A gene than previous studies, indicating its potential involvement in degrading aromatic hydrocarbons through anaerobic biochemical pathways in oil fields. The bam A gene sequences were affiliated to bacteria that are known to degrade aromatics, including Azoarcus, Georgfuchsia, Sulfuritalea, Peptococcaceae and Syntrophus . Differences of composition and diversity of bam A gene sequences over a period of four months in these oil reservoirs showed that it is necessary to monitor functional genes over a period of time to fully establish their significance to any specific process at any site. Highlights: The diversity of bam A genes found in oil reservoir produced water was higher than previous reports. The bam A genes related to nitrate reducing bacteria indicates the potential for Microbial Enhanced Oil Recovery application. Firmicutes ( Desulfotomaculum spp.) may be important aromatic hydrocarbons degraders in both oil wells.