Amphiphilic poly-N-vynilpyrrolidone nanoparticles: Cytotoxicity and acute toxicity study. (October 2016)

• Record Type: Journal Article
• Authors: Kuskov, A.N.; Kulikov, P.P.; Shtilman, M.I.; Rakitskii, V.N.; Tsatsakis, A.M.
• Is Part Of: Food and chemical toxicology. Volume 96(2016)
• Page Start: 273
• Page End: 279
• Publication Date: 2016-10
• Subjects: Poly-N-vinylpyrrolidone -- Amphiphilic -- Nanoparticle -- Cytotoxicity -- Acute toxicity -- Drug delivery system; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.08.017
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The aim of the present study was to evaluate the cytotoxicity against MCF-7 cells and acute intraperitoneal toxicity of amphiphilic poly- N -vinylpyrrolidone nanoparticles to confirm possibility of their application for creation of novel drug delivery systems. The effect of cellular uptake of polymeric nanoparticles on human cancer cell line MCF-7 cells was investigated by MTT assay. MTT analysis showed that tested amphiphilic polymers were essentially non-toxic. In acute toxicity studies, LD50 and other toxicity indexes were evaluated, under which no deaths or treatment related complications were observed even in high concentration treatment for 14 days of experiment. For histological analysis, organs of the animals were weighed and examined. No animal died during the study and no significant changes have been observed regarding body weight, feed consumption, organ weight or histological data. Obtained results show that amphiphilic poly- N -vinylpyrrolidone nanoparticles possessed no toxicity against cells and in animals after intraperitoneal administration. Thus, amphiphilic PVP nanoparticles demonstrate high potential as carriers for novel high-effective drug delivery systems. Graphical abstract: Highlights: Amphiphilic poly-N-vinylpyrrolidone nanoparticles were prepared and characterized. No cytotoxicity was shown for the NVP-based polymeric nanoparticles against MCF-7 cells in MTT assay. No acute toxicity was demonstrated for the NVP-based polymeric nanoparticles after single intraperitoneal administration in mice and rats.