The effect of silver nanoparticles and silver ions on mammalian and plant cells in vitro. (October 2016)

• Record Type: Journal Article
• Authors: Jiravova, Jana; Tomankova, Katerina Barton; Harvanova, Monika; Malina, Lukas; Malohlava, Jakub; Luhova, Lenka; Panacek, Ales; Manisova, Barbora; Kolarova, Hana
• Is Part Of: Food and chemical toxicology. Volume 96(2016)
• Page Start: 50
• Page End: 61
• Publication Date: 2016-10
• Subjects: Silver nanoparticles -- Silver ions -- In vitro cytotoxicity -- Genotoxicity -- Solanum spp. -- Mammalian cell; ROS reactive oxygen species -- DMEM Dulbecco's modified Eagle's medium -- FBS fetal bovine serum -- IC50 50% lethal concentration -- AgNPs silver nanoparticles -- AFM atomic force microscopy -- RT room temperature -- DLS dynamic light scattering -- TEM transmission electron microscopy -- LPOX lipid peroxidation -- POX peroxidase; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.07.015
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Silver nanoparticles (AgNPs) are the most frequently applied nanomaterials. In our experiments, we tested AgNPs (size 27 nm) manufactured by the Tollens process. Physico-chemical methods (TEM, DLS, AFM and spectrophotometry) were used for characterization and imaging of AgNPs. The effects of AgNPs and Ag + were studied in two experimental models (plant and mammalian cells). Human keratinocytes (SVK14) and mouse fibroblasts (NIH3T3) cell lines were selected to evaluate the cytotoxicity and genotoxicity effect on mammalian cells. Higher sensitivity to AgNPs and Ag + was observed in NIH3T3 than in SVK14 cells. AgNPs accumulated in the nucleus of NIH3T3 cells, caused DNA damage and increased the number of apoptotic and necrotic cells. Three genotypes of Solanum spp. ( S. lycopersicum cv. Amateur, S. chmielewskii, S. habrochaites ) were selected to test the toxicity of AgNPs and Ag + on the plant cells. The highest values of peroxidase activity and lipid peroxidation were recorded after the treatment of S. habrochaites genotype with AgNPs. Increased ROS levels were likely the reason for observed damaged membranes in S. habrochaites . We found that the cytotoxic and genotoxic effects of AgNPs depend not only on the characteristics of nanoparticles, but also on the type of cells that are treated with AgNPs. Highlights: Study of the effect of AgNPs and Ag + in plant and mammalian cells. AgNPs accumulated in the nucleus of NIH3T3 cells and caused significant DNA damage and cell apoptosis. AgNPs caused oxidative stress in Solanum spp. The cytotoxic and genotoxic effect of AgNPs depends on the type of cells.