Predicting human intestinal absorption in the presence of bile salt with micellar liquid chromatography. (1st May 2016)

• Record Type: Journal Article
• Authors: Waters, Laura J.; Shokry, Dina S.; Parkes, Gareth M.B.
• Is Part Of: Biomedical chromatography. Volume 30:Number 10(2016:Oct.)
• Page Start: 1618
• Page End: 1624
• Publication Date: 2016-05-01
• Subjects: intestinal absorption -- chromatography -- MLC -- micellar -- bile salts; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3731
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Understanding intestinal absorption for pharmaceutical compounds is vital to estimate the bioavailability and therefore the in vivo potential of a drug. This study considers the application of micellar liquid chromatography (MLC) to predict passive intestinal absorption with a selection of model compounds. MLC is already known to aid prediction of absorption using simple surfactant systems; however, with this study the focus was on the presence of a more complex, bile salt surfactant, as would be encountered in the in vivo environment. As a result, MLC using a specific bile salt has been confirmed as an ideal in vitro system to predict the intestinal permeability for a wide range of drugs, through the development of a quantitative partition–absorption relationship. MLC offers many benefits including environmental, economic, time‐saving and ethical advantages compared with the traditional techniques employed to obtain passive intestinal absorption values. Copyright © 2016 John Wiley & Sons, Ltd.