High Spatial Resolution Imaging of Endogenous Hydrogen Peroxide in Living Cells by Solid‐State Fluorescence. (13th July 2016)

• Record Type: Journal Article
• Authors: Lindberg, Eric; Winssinger, Nicolas
• Is Part Of: Chembiochem. Volume 17:Number 17(2016)
• Page Start: 1612
• Page End: 1615
• Publication Date: 2016-07-13
• Subjects: fluorescent probes -- hydrogen peroxide -- imaging -- precipitating dye -- quinazolinone; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201600211
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Herein, we describe selective imaging of hydrogen peroxide using a precipitating dye conjugated to a boronic acid‐based immolative linker. We achieved visualization of endogenous hydrogen peroxide in phagosomes by solid‐state two‐photon fluorescence imaging in living cells with exceptionally high spatial resolution. Abstract : H2 O2 w it works : Selective imaging of hydrogen peroxide in phagosomes was achieved by using a precipitating dye and a boronic acid‐based linker. Using this method, we were able to visualize endogenous hydrogen peroxide by solid‐state two‐photon fluorescence imaging in living cells with exceptionally high spatial resolution.