Expression of nephronectin is enhanced by 1α, 25‐dihydroxyvitamin D3. Issue 9 (13th July 2016)

• Record Type: Journal Article
• Authors: Hiranuma, Katsuhiro; Yamada, Atsushi; Kurosawa, Tamaki; Aizawa, Ryo; Suzuki, Dai; Saito, Yoshiro; Nagahama, Ryo; Ikehata, Mikiko; Tsukasaki, Masayuki; Morimura, Naoko; Chikazu, Daichi; Maki, Koutaro; Shirota, Tatsuo; Takami, Masamichi; Yamamoto, Matsuo; Iijima, Takehiko; Kamijo, Ryutaro
• Is Part Of: FEBS open bio. Volume 6:Issue 9(2016)
• Page Start: 914
• Page End: 918
• Publication Date: 2016-07-13
• Subjects: 1α, 25‐dihydroxyvitamin D3 -- nephronectin -- vitamin D receptor; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.12085
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : The extracellular matrix protein nephronectin (Npnt), also called POEM, is considered to play critical roles as an adhesion molecule in development and functions of various tissues, such as the kidneys, liver, and bone. In the present study, we examined the molecular mechanism of Npnt gene expression and found that vitamin D3 (1α, 25‐dihydroxyvitamin D3, VD3 ) strongly enhanced Npnt mRNA expression in MC3T3‐E1 cells from a mouse osteoblastic cell line. The VD3 ‐induced increase in Npnt expression is both time‐ and dose‐dependent and is mediated by the vitamin D receptor (VDR). Abstract : Expression of cell adhesion molecule nephronectin (Npnt) is induced by 1α, 25‐dihydroxyvitamin D3 (VD3 ) through vitamin D receptor (VDR). In this proposed model, VD3 binding to VDR forms a heterodimer with retinoid X receptor (RXR), which may bind to vitamin D responsible elements (VDRE) in the hypothetical regulatory regions of the Npnt gene to regulate its expression.