A ZnO–CNT nanocomposite based electrochemical DNA biosensor for meningitis detection. Issue 80 (11th August 2016)

• Record Type: Journal Article
• Authors: Tak, Manvi; Gupta, Vinay; Tomar, Monika
• Is Part Of: RSC advances. Volume 6:Issue 80(2016)
• Page Start: 76214
• Page End: 76222
• Publication Date: 2016-08-11
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra12453d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The article focuses on the synthesis of ZnO and CNTs based electrochemical DNA biosensor and its application towards meningitis DNA detection with high sensitivity as well as selectivity. Abstract : A zinc oxide (ZnO) and multiwalled carbon nanotube (CNT) based nanocomposite matrix has been fabricated onto indium tin oxide (ITO) coated glass (ITO/glass) platform via . A chemical route. The thiolated oligonucleotides probe sequence consisting of 23 bases (single-stranded DNA) has been immobilized onto the ZnO–CNT/ITO electrode using a physical adsorption technique. The fabricated bioelectrode (ssDNA/ZnO–CNT/ITO) is used to specifically detect N. meningitidis with a high sensitivity of 20.20 μA per decade and 45 s of hybridization time by differential pulse voltammetry (DPV) using methylene blue as an electroactive indicator. The prepared DNA biosensor exhibits linearity in a wide range (5–180 ng μl −1 ). The electrochemical impedance response behaviour of the ssDNA/ZnO–CNT/ITO bioelectrode with an increase in the target DNA concentration has also been studied using [Fe(CN)6 ] 3−/4− as the hybridization indicator. The results indicate the possibility of fabricating a handheld device for meningitis detection at an early stage.