Morphological stability of microencapsulated vitamin formulations by AFM imaging. (July 2016)

• Record Type: Journal Article
• Authors: Mougin, Karine; Bruntz, Adèle; Severin, Delphine; Teleki, Alexandra
• Is Part Of: JPRAS open. Volume 9(2016)
• Page Start: 1
• Page End: 12
• Publication Date: 2016-07
• Subjects: Atomic force microscopy -- Microencapsulation -- Vitamin -- Formulation -- Emulsion -- Surface roughness parameters; Surgery, Plastic -- Great Britain -- Periodicals; 617.9505
• DOI: 10.1016/j.foostr.2016.07.001
• Languages: English
• ISSNs: 2352-5878

Abstract

Graphical abstract: Highlights: Lipophilic vitamins were formulated in microencapsulated product forms. Internal particle morphology of Vitamin A formulations was visualized by AFM. Changes in this morphology by increased temperature or humidity were evaluated. The morphological stability was affected by matrix composition. Morphological stability was predicted with accelerated AFM stress tests. Abstract: The morphological stability of microencapsulated lipophilic vitamin formulations directly influences the long-term storage stability and performance in final applications. The internal particle morphology of three commercial dry Vitamin A acetate formulations differing in their matrix composition (hydrocolloid/polysaccharide and plasticizer) was systematically characterized by atomic force microscopy (AFM). The evolution of the particle internal morphology after per-se storage at 25 and 40 °C for ten months was investigated. Furthermore, an accelerated stress test by in-situ heating and AFM imaging was developed. Surface roughness parameters from the AFM images were used to semi-quantitatively estimate the evolution of the particle internal microstructure, and thus the aging and destabilization of the lipophilic vitamin formulations. The influence of humidity level on the thermal morphological restructuring of one of the commercial samples was also investigated. This study has shown that such AFM investigations of microparticles are a novel analytical tool to rapidly estimate the physical stability of the lipophilic vitamin dried emulsions.