3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy. Issue 67 (29th July 2016)

• Record Type: Journal Article
• Authors: Pan, Bin-Bin; Yang, Feng; Ye, Yansheng; Wu, Qiong; Li, Conggang; Huber, Thomas; Su, Xun-Cheng
• Is Part Of: Chemical communications. Volume 52:Issue 67(2016)
• Page Start: 10237
• Page End: 10240
• Publication Date: 2016-07-29
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc05490k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The integration of site-specific labeling of proteins with a stable lanthanide binding tag, paramagnetic NMR spectroscopy and the GPS-Rosetta program presents an effective and fast way of determining the three-dimensional structure of a protein in living cells. Abstract : Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein–ligand complexes.