Invitro Bioactivity and Osteogenic Activity Study of Solid State Synthesized Nano‐Hydroxyapatite using Recycled Eggshell Bio–waste. Issue 13 (30th August 2016)

• Record Type: Journal Article
• Authors: Ingole, Vijay H.; Hussein, Kamal H.; Kashale, Anil A.; Gattu, Ketan P.; Dhanayat, Swapnali S.; Vinchurkar, Aruna; Chang, Jia‐Yaw; Ghule, Anil V.
• Is Part Of: ChemistrySelect. Volume 1:Issue 13(2016)
• Page Start: 3901
• Page End: 3908
• Publication Date: 2016-08-30
• Subjects: Bioactivity -- Eggshell bio-waste -- Hydroxyapatite -- Orthopaedic -- Osteogenicity -- Solid state synthesis; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201601092
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Research on hydroxyapatite is experiencing a remarkable increase during the recent years due to its potential biological and biomedical applications. Herein, we have synthesized nano‐hydroxyapatite (HAp) by solid state reaction (SSR), wherein recycled eggshell bio‐waste as Ca precursor on processing is reacted with synthetic ammonium dihydrogen orthophosphate to form hydroxyapatite (SSHAp). The solid state synthesis reaction is studied by thermogravimetric analysis. The morphology and crystal structure of SSHAp nanoparticles is characterized and found to be spherical and free of aggregation with average crystallite size ∼ 10 nm. The SSHAp nanoparticles show large surface area (9.64 m 2 g −1 ). Furthermore, the invitro bioactivity and osteogenic activity of SSHAp is investigated. The osteogenic activity is evaluated by incubating the SSHAp with hFOB cells. The SSHAp is observed to promote appreciable cell attachment and proliferation unlike in control material. While hFOB cells is found to express higher level of osteocalcin, osteopontin, collagen I, osteonectin, BMP‐2 and GAPDH on the SSHAp bone graft. The results show that SSHAp nanoparticles graft is bioactive, nontoxic and osteogenic, and has the potential to promote bone therapy applications. The HAp is synthesized using recycled bio‐waste contributing to green chemistry, addressing the environmental concerns of recycle and reuse. Abstract : SSHAp is synthesized by solid state reaction (SSR) using recycled eggshell bio‐waste contributing to green chemistry principle of recycle and reuse. The SSR reaction is studied by TGA. The nanoparticles are spherical, free of aggregation with average crystallite size ∼ 10 nm and show large surface area (9.64 m 2 g −1 ). Its invitro bioactivity and osteogenic activity is investigated. The results provide the evidence that SSHAp nanoparticles graft is bioactive, nontoxic and osteogenic, and has the potential to promote bone therapy applications.