Substrate Pre‐Folding and Water Molecule Organization Matters for Terpene Cyclase Catalyzed Conversion of Unnatural Substrates. Issue 13 (19th August 2016)

• Record Type: Journal Article
• Authors: Hammer, Stephan C.; Syrén, Per‐Olof; Hauer, Bernhard
• Is Part Of: ChemistrySelect. Volume 1:Issue 13(2016)
• Page Start: 3589
• Page End: 3593
• Publication Date: 2016-08-19
• Subjects: biocatalysis -- enzyme catalysis -- promiscuity -- squalene hopene cyclase -- terpene cyclases; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201600572
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Terpene cyclase enzymes have recently been challenged with terpene substrate derivatives to generate additional chemical complexity beyond to what is currently found in nature. Herein, molecular dynamics and biocatalysis are used to shed light on the flexibility and inherent limitation of a triterpene cyclase in converting unnatural substrates. Our studies suggest that populating binding modes which allows for concerted reaction pathways is a key element towards an expanded substrate scope and new chemistries displayed by terpene cyclases. Additionally, we show that the spatial organization of water, which is influenced by both the substrate architecture as well as the active site geometry, controls the product selectivity. This highlights that activity and selectivity displayed by terpene cyclases acting on unnatural substrates is particularly difficult to predict, since they depend on various parameters. Abstract : Terpene cyclase enzymes have recently been challenged with substrate derivatives to generate additional chemical complexity beyond to what is found in nature. Herein, molecular dynamics and biocatalysis are used to shed light on the flexibility and inherent limitation of a triterpene cyclase in converting unnatural substrates.