Efficient purification and complete NMR characterization of galactinol, sucrose, raffinose, and stachyose isolated from Pinus halepensis (Aleppo pine) seeds using acetylation procedure. Issue 4 (3rd May 2016)

• Record Type: Journal Article
• Authors: Ben Youssef, Sahar; Fakhfakh, Jawhar; Tchoumtchoua, Job; Halabalaki, Maria; Allouche, Noureddine
• Is Part Of: Journal of carbohydrate chemistry. Volume 35:Issue 4(2016)
• Page Start: 224
• Page End: 237
• Publication Date: 2016-05-03
• Subjects: Acetylated galactinol -- acetylated sucrose -- acetylated raffinose -- acetylated stachyose -- NMR characterization -- Pinus halepensis; Carbohydrates -- Periodicals; 547.78
• DOI: 10.1080/07328303.2016.1207781
• Languages: English
• ISSNs: 0732-8303

Abstract

GRAPHICAL ABSTRACT: ABSTRACT: The use of precipitation followed by acetylation procedures and preparative TLC purification allowed a facile isolation of four carbohydrates from the methanol extract of Pinus halepensis seeds. The isolated oligosaccharides exhibited high degree of purity. They were identified as α-D-galactosyl-(1→1)- myo -inositol nonaacetate (1 ), α-D-glucosyl-(1→2)-β-D-fructosyl octaacetate (2 ), α-D-galactosyl-(1→6)-α-D-glucosyl-(1→2)-β-D-frutosyl undecaacetate (3 ), and α-D-galactosyl-(1→6)-α-D-galactosyl-(1→6)-α-D-glucosyl-(1→2)-β-D-frutosyl tetradecaacetate (4 ) and were isolated for the first time from this plant. The 1 H and 13 C NMR assignments for compounds2, 3, and4 were detailed herein for the first time.