Muropeptides in Pseudomonas aeruginosa and their Role as Elicitors of β‐Lactam‐Antibiotic Resistance. Issue 24 (25th April 2016)

• Record Type: Journal Article
• Authors: Lee, Mijoon; Dhar, Supurna; De Benedetti, Stefania; Hesek, Dusan; Boggess, Bill; Blázquez, Blas; Mathee, Kalai; Mobashery, Shahriar
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 24(2016)
• Page Start: 6882
• Page End: 6886
• Publication Date: 2016-04-25
• Subjects: β-lactamase -- antibiotic resistance -- bacteria -- muropeptides -- peptidoglycan; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201601693
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Muropeptides are a group of bacterial natural products generated from the cell wall in the course of its turnover. These compounds are cell‐wall recycling intermediates and are also involved in signaling within the bacterium. However, the identity of these signaling molecules remains elusive. The identification and characterization of 20 muropeptides from Pseudomonas aeruginosa is described. The least abundant of these metabolites is present at 100 and the most abundant at 55, 000 molecules per bacterium. Analysis of these muropeptides under conditions of induction of resistance to a β‐lactam antibiotic identified two signaling muropeptides ( N ‐acetylglucosamine‐1, 6‐anhydro‐ N ‐acetylmuramyl pentapeptide and 1, 6‐anhydro‐ N ‐acetylmuramyl pentapeptide). Authentic synthetic samples of these metabolites were shown to activate expression of β‐lactamase in the absence of any β‐lactam antibiotic, thus indicating that they serve as chemical signals in this complex biochemical pathway. Abstract : Muropeptides : A total of 20 muropeptides, which are breakdown products of peptidoglycan, were isolated from Pseuromonas aeruginosa and their structures were elucidated. The least abundant of these metabolites is present at 100 and the most abundant at 55 000 molecules per bacterium. Two of these compounds were shown to be involved in the induction of β‐lactamase expression. This effect is the basis for resistance to β‐lactam antibiotics in P. aeruginosa .