Nonstimulated rabbit phonation model: Cricothyroid approximation. (12th March 2016)

• Record Type: Journal Article
• Authors: Novaleski, Carolyn K.; Kojima, Tsuyoshi; Chang, Siyuan; Luo, Haoxiang; Valenzuela, Carla V.; Rousseau, Bernard
• Is Part Of: Laryngoscope. Volume 126:Number 7(2016:Jul.)
• Page Start: 1589
• Page End: 1594
• Publication Date: 2016-03-12
• Subjects: Rabbit model -- phonation -- vocal fold; Otolaryngology -- Periodicals; 617.51005
• DOI: 10.1002/lary.25559
• Languages: English
• ISSNs: 0023-852X

Abstract

Abstract : Objectives/Hypothesis: To describe a nonstimulated in vivo rabbit phonation model using an Isshiki type IV thyroplasty and uninterrupted humidified glottal airflow to produce sustained audible phonation. Study Design: Prospective animal study. Methods: Six New Zealand white breeder rabbits underwent a surgical procedure involving an Isshiki type IV thyroplasty and continuous airflow delivered to the glottis. Phonatory parameters were examined using high‐speed laryngeal imaging and acoustic and aerodynamic analysis. Following the procedure, airflow was discontinued, and sutures remained in place to maintain the phonatory glottal configuration for microimaging using a 9.4 Tesla imaging system. Results: High‐speed laryngeal imaging revealed sustained vocal fold oscillation throughout the experimental procedure. Analysis of acoustic signals revealed a mean vocal intensity of 61 dB and fundamental frequency of 590 Hz. Aerodynamic analysis revealed a mean airflow rate of 85.91 mL/s and subglottal pressure of 9 cm H2 O. Following the procedure, microimaging revealed that the in vivo phonatory glottal configuration was maintained, providing consistency between the experimental and postexperimental laryngeal geometry. The latter provides a significant milestone that is necessary for geometric reconstruction and to allow for validation of computational simulations against the in vivo rabbit preparation. Conclusion: We demonstrate a nonstimulated in vivo phonation preparation using an Isshiki type IV thyroplasty and continuous humidified glottal airflow in a rabbit animal model. This preparation elicits sustained vocal fold vibration and phonatory measures that are consistent with our laboratory's prior work using direct neuromuscular stimulation for evoked phonation. Level of Evidence: N/A. Laryngoscope, 126:1589–1594, 2016