Circulating microRNAs and sudden cardiac arrest outcomes. (September 2016)

• Record Type: Journal Article
• Authors: Wander, P.L.; Enquobahrie, D.A.; Pritchard, C.C.; McKnight, B.; Rice, K.; Christiansen, M.; Lemaitre, R.N.; Rea, T.; Siscovick, D.; Sotoodehnia, N.
• Is Part Of: Resuscitation. Volume 106(2016)
• Page Start: 96
• Page End: 101
• Publication Date: 2016-09
• Subjects: Cardiac arrest -- Outcomes -- Epigenetics -- microRNAs -- Epidemiology; Resuscitation -- Periodicals; Resuscitation -- Periodicals; Réanimation -- Périodiques; Electronic journals; 616.025
• DOI: 10.1016/j.resuscitation.2016.06.038
• Languages: English
• ISSNs: 0300-9572

Abstract

Abstract: Aim: MicroRNAs (miRNAs) have regulatory functions in organs critical in resuscitation from sudden cardiac arrest due to ventricular fibrillation (VF-SCA); therefore, circulating miRNAs may be markers of VF-SCA outcome. Methods: We measured candidate miRNAs ( N = 45) in plasma using qRT-PCR among participants of a population-based VF-SCA study. Participants were randomly selected cases who died in the field (DF, n = 15), died in hospital (DH, n = 15), or survived to discharge (DC, n = 15), and, age-, sex-, and race-matched controls ( n = 15). MiRNA levels were compared using ANOVA, t -tests, and fold-changes. Results: Mean age of groups ranged from 66.9 to 69.7. Most participants were male (53–67%) and white (67%). Comparing cases to controls, plasma levels of 17 miRNAs expressed in heart, brain, liver, and other tissues (including miR-29c, -34a, -122, -145, -200a, -210, -499-5p, and -663b) were higher and three non-specific miRNAs lower (miR-221, -330-3p, and -9-5p). Among DH or DC compared with DF cases, levels of two miRNAs (liver-specific miR-122 and non-specific miR-205) were higher and two heart-specific miRNAs (miR-208b and -499-5p) lower. Among DC vs. DF cases, levels of three miRNAs (miR-122, and non-specific miR-200a and -205) were higher and four heart-specific miRNAs (miR-133a, -133b, -208b, and -499-5p) lower. Among DC vs. DH cases, levels of two non-specific miRNAs (miR-135a and -9-3p) were lower. Conclusions: Circulating miRNAs expressed in heart, brain, and other tissues differ between VF-SCA cases and controls and are related to resuscitation outcomes. Measurement of miRNAs may clarify mechanisms underlying resuscitation, improve prognostication, and guide development of therapies. Results require replication.