Mur (MNS 10) screening with a novel loop‐mediated isothermal amplification assay in Zhongshan, China. Issue 3 (22nd April 2016)

• Record Type: Journal Article
• Authors: Wei, S.S.; Sun, A.N.; Ding, S.H.; Meng, Q.L.; Wang, H.M.; Duan, S.B.; Tian, J.J.; Chen, Y.Z.; Liu, X.M.; Li, Y.; Liao, Y.T.
• Is Part Of: Transfusion medicine. Volume 26:Issue 3(2016:Jun.)
• Page Start: 215
• Page End: 219
• Publication Date: 2016-04-22
• Subjects: GP. Mur -- loop‐mediated isothermal amplification -- Miltenberger -- molecular diagnosis; Blood -- Transfusion -- Periodicals; 615.39
• DOI: 10.1111/tme.12305
• Languages: English
• ISSNs: 0958-7578

Abstract

ABSTRACT: Background: The distribution of the Mur blood group antigen is 5–7% in the south of China, and a much higher prevalence is observed in some areas of the region. Anti‐Mur can cause hemolytic disease of the newborn and severe transfusion reactions. Objectives: Genetic testing is more ideal than conventional serological tests because antibodies for detection are usually not available. Methods: In this study, a novel loop‐mediated isothermal amplification (LAMP) assay for the detection of Mur blood group antigen was established. Results: Fifteen of 275 (5·5%) samples were confirmed by LAMP as Mur antigen positive. All the Mur antigen‐positive samples were GP.Mur subtype which was confirmed with sequencing. Conclusion: The LAMP method has identical results with conventional serology method but more suitable for large‐scale screening.