The Effects of the Low Temperature Argon Plasma on Stem Cells Proliferation and Regeneration in Planarians. Issue 8 (18th April 2016)

• Record Type: Journal Article
• Authors: Ermakov, Artem; Ermakova, Olga; Skavulyak, Alexander; Kreshchenko, Natalia; Gudkov, Sergey; Maevsky, Eugene
• Is Part Of: Plasma processes and polymers. Volume 13:Issue 8(2016)
• Page Start: 788
• Page End: 801
• Publication Date: 2016-04-18
• Subjects: cold plasma -- gene expression -- planarian -- regeneration -- stem cells proliferation; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.201500203
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract : Effects of the low temperature argon plasma (LTAP) in intact and regenerating planarians are studied. The LTAP actions were specified as activating or inhibiting using stem cells proliferation and the regenerative blastema growth, and the expression level of wound‐specific genes responsible for regeneration. The activating mode of LTAP treatment does not induce the generation of the Reactive Oxygen Species (ROS) but the inhibiting mode of LTAP treatment causes the oxidative stress response in planarian tissue without DNA damage. The results indicate that LTAP wound healing action in planarians could be similar in cellular and molecular mechanisms of the higher organisms including humans. The effects of LTAP irradiation as a factor of the low intensity are discussed from standpoint of the hormesis hypothesis. Abstract : Using "in vivo" flatworms (planarians) biological model we show for the first time that the low temperature argon plasma (the LTAP) stimulates or inhibits planarian regeneration process depending from the modes of action applied. It is found that observable LTAP effects are mediated by its impact on the genes expression and the proliferation of planarian stem cells, the neoblasts.