A μs‐Pulsed Dielectric Barrier Discharge Source: Physical Characterization and Biological Effects on Human Skin Fibroblasts. Issue 8 (29th January 2016)

• Record Type: Journal Article
• Authors: Tiede, Regina; Hirschberg, Joanna; Viöl, Wolfgang; Emmert, Steffen
• Is Part Of: Plasma processes and polymers. Volume 13:Issue 8(2016)
• Page Start: 775
• Page End: 787
• Publication Date: 2016-01-29
• Subjects: atmospheric pressure plasma -- dielectric barrier discharge -- medical plasma devices -- safety evaluation; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.201500190
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract : As the field of plasma medicine eventually develops, safety aspects of biocompatible plasma sources are becoming ever more important for a safe usage of plasma devices as medical instruments. In this study physical and biological safety parameters of an atmospheric pressure dielectric barrier discharge (DBD) source with a phosphate buffered saline‐electrode (PBS‐electrode) were analyzed. For a general characterization of this DBD device the dissipated power, rotational and electron energies, and reactive species were investigated. Cell vitality tests of human skin fibroblasts treated with the device directly and indirectly were performed using an MTS assay. Additionally, applying a host cell reactivation (HCR) assay fibroblasts' DNA repair capacity for plasma‐induced damages was examined. Our risk assessments indicate that this DBD device can be used safely in therapeutic applications. Abstract : Biocompatible atmospheric pressure plasma is a new therapeutic approach in medicine with a variety of possible application fields. Especially for treatments of dermatological diseases atmospheric pressure plasma seems to be beneficial. In this article a new μs‐pulsed dielectric barrier discharge with a phosphate buffered saline‐electrode is introduced. Furthermore, we provide a general physical characterization of the device, as well as a risk assessment of post‐plasma biological effects on human skin fibroblasts and effects on DNA. Resulting data are discussed regarding safety aspects for medical use.