Effects of PTEN inhibition on the regulation of Tau phosphorylation in rat cortical neuronal injury after oxygen and glucose deprivation. (28th July 2016)

• Record Type: Journal Article
• Authors: Zhao, Jing; Chen, Yurong; Xu, Yuxia; Pi, Guanghuan
• Is Part Of: Brain injury. Volume 30:Number 9(2016)
• Page Start: 1150
• Page End: 1159
• Publication Date: 2016-07-28
• Subjects: Neuron -- hypoxia-ischaemia -- oxygen and glucose deprivation -- signalling pathway -- PTEN -- tau -- Akt; Brain damage -- Periodicals; Brain -- Wounds and injuries -- Periodicals; Brain Injuries -- Periodicals; 617.481
• DOI: 10.3109/02699052.2016.1161828
• Languages: English
• ISSNs: 0269-9052

Abstract

Abstract: Objective : This report investigated the involvement of the PTEN pathway in the regulation of Tau phosphorylation using an oxygen and glucose deprivation (OGD) model with rat cortical neurons. Methods : Primary cortical neurons were used to establish the oxygen and glucose deprivation (OGD) model in vitro . These were randomly divided into control, OGD, bpV+OGD, As+OGD, Se+OGD and Mock treatment groups. The neuron viability was assessed by MTT, the cell apoptosis was detected using TUNEL staining. The expression of Phospho-PTEN/PTEN, Phospho-Tau/Tau, Phospho-Akt/Akt and Phospho-GSK-3 β /GSK-3 β were detected by Western blotting. Results : OGD induced Tau phosphorylation through PTEN and glycogen synthase kinase-3 β (GSK-3 β ) activation, together with a decrease in AKT activity. Pre-treatment with bpv, a potent PTEN inhibitor, and PTEN antisense nucleotides decreased PTEN and GSK-3 β activity and caused alterations in Tau phosphorylation. Neuronal apoptosis was also reduced. Conclusions : The PTEN/Akt/GSK-3 β /Tau pathway is involved in the regulation of neuronal injury, providing a novel route for protecting neurons following neonatal HI.