Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata). (18th May 2016)

• Record Type: Journal Article
• Authors: He, Shengjie; Mao, Xinliang; Zhang, Ting; Guo, Xiaolei; Ge, Yazhong; Ma, Chungwah; Zhang, Xuewu
• Is Part Of: Journal of microencapsulation. Volume 33:Number 4(2016:Jun.)
• Page Start: 344
• Page End: 354
• Publication Date: 2016-05-18
• Subjects: Cuora trifasciata -- hydrolysis -- peptides -- antitumor -- nanoencapsulation; Microencapsulation -- Periodicals; Controlled release technology -- Periodicals; 615.19
• DOI: 10.1080/02652048.2016.1194904
• Languages: English
• ISSNs: 0265-2048

Abstract

Abstract: Chinese three-striped box turtle ( Cuora trifasciata ), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65–89.1%, at 500 μg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1–1), KLGPKGPR (T1–2), and SSPGPPVH (T2–1). By database search, T2–1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 μg/mL. Then, T1 and T2–1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2–1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice.