Adipose-derived stem cells sustain prolonged angiogenesis through leptin secretion. (3rd July 2016)

• Record Type: Journal Article
• Authors: Delle Monache, Simona; Calgani, Alessia; Sanità, Patrizia; Zazzeroni, Francesca; Gentile Warschauer, Emilio; Giuliani, Antonio; Amicucci, Gianfranco; Angelucci, Adriano
• Is Part Of: Growth factors. Volume 34:Number 3/4(2016)
• Page Start: 87
• Page End: 96
• Publication Date: 2016-07-03
• Subjects: Angiogenesis -- growth factors -- endothelial progenitor cells -- CD105; Growth factors -- Periodicals; Physiology -- Periodicals; 611
• DOI: 10.1080/08977194.2016.1191481
• Languages: English
• ISSNs: 0897-7194

Abstract

Abstract: Recent studies suggest that adipose-derived stem cells (ASCs) play a role in tissue remodeling through the release of cytokines and growth factors. We compared the secreted cytokine profile of hypoxia-conditioned ASCs (hASCs) with normoxic ASCs (nASCs) and we analyzed the effect of ASCs conditioned medium (CM) on endothelial cells. We found that hypoxia induced a transient upregulation of VEGF in ASCs and a notable and enduring upregulation of leptin mRNA expression 30-fold greater than control after 24 h and up to 60-fold greater than control at day 7. CM from hASC stimulated EC tube formation to a significantly greater extent than CM from nASC. This might be due to leptin-secreted factor. Indeed, exogenous leptin stimulated the expression of HIF2-α, but not HIF1-α, and upregulated the expression of Flt-1 and Tie-1 proangiogenic receptors. In conclusion, hASCs may be particularly efficient in sustaining angiogenesis through the release of leptin.