7SKiing on chromatin: Move globally, act locally. Issue 6 (2nd June 2016)

• Record Type: Journal Article
• Authors: D'Orso, Iván
• Is Part Of: RNA biology. Volume 13:Issue 6(2016)
• Page Start: 545
• Page End: 553
• Publication Date: 2016-06-02
• Subjects: KAP1/TRIM28 -- P-TEFb -- RNA polymerase II -- 7SK snRNP -- transcription elongation; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2016.1181254
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: RNA polymerase II (Pol II) pausing at promoter-proximal regions is a highly regulated step in the transcription cycle. Pause release is facilitated by the P-TEFb kinase, which phosphorylates Pol II and negative elongation factors. Recent studies suggest that P-TEFb (as part of the inhibitory 7SK snRNP) is recruited to promoter-proximal regions through interaction with KAP1/TRIM28/TIF1β to facilitate 'on-site' kinase activation and transcription elongation. Here, I discuss features of this model and future challenges to further hone our understanding of transcriptional regulation including Pol II pausing and pause release.