Regulation of eIF2α expression and renal interstitial fibrosis by resveratrol in rat renal tissue after unilateral ureteral obstruction. (20th April 2016)

• Record Type: Journal Article
• Authors: Zhang, Cui; Zhou, Yanyan; Zhou, Yajie; Lu, Ying; Wang, Danfeng
• Is Part Of: Renal failure. Volume 38:Number 4(2016)
• Page Start: 622
• Page End: 628
• Publication Date: 2016-04-20
• Subjects: ATF-4 -- eIF2α -- endoplasmic reticulum stress -- renal interstitial fibrosis -- resveratrol; Chronic renal failure -- Periodicals; Acute renal failure -- Periodicals; Uremia -- Periodicals; 616.614005
• DOI: 10.3109/0886022X.2016.1149774
• Languages: English
• ISSNs: 0886-022X

Abstract

Abstract: Purpose This study was performed to assess the effect of resveratrol on the expression of eukaryotic initiation factor 2α (eIF2α) and activating transcription factor 4 (ATF4) in renal tissues of rats with unilateral ureteral obstruction (UUO).Methods Using UUO animal model, after 14 days of surgery, pathological changes were detected by HE staining, renal tubular damage index, renal interstitial collagen deposition area were evaluated by Masson staining, in situ cell apoptosis in renal tissue was analyzed by TUNEL assay, and protein expression of eIF2α and ATF4 in renal tissue was analyzed using western blot detection.Results After comparison of the treatment groups with model group, we observed that the degree of renal tubular damage, relative area of renal interstitial collagen and eIF2α, ATF4 protein expression were also significantly reduced ( p< 0.05, p < 0.01) in the high-dose resveratrol group.Conclusion Resveratrol can reduce the level of eIF2α protein expression, which further reduces the ATF4 levels.