Impacts of β-estradiol on intestinal injury in newborn rats. (17th June 2016)

• Record Type: Journal Article
• Authors: Kilicdag, Hasan; Daglioglu, Yusuf Kenan; Sencar, Leman; Erdogan, Seyda; Zorludemir, Suzan; Polat, Sait; Bagir, Emine Kilic; Coskun, Gulfidan
• Is Part Of: Journal of maternal-fetal & neonatal medicine. Volume 29:Number 12(2016)
• Page Start: 1935
• Page End: 1940
• Publication Date: 2016-06-17
• Subjects: β-Estradiol -- IL-6 -- intestinal injury -- necrotizing enterocolitis -- newborn rat -- TNF-α; Obstetrics -- Periodicals; Perinatology -- Periodicals; Infants (Newborn) -- Diseases -- Periodicals; Neonatology -- Periodicals; 618.2
• DOI: 10.3109/14767058.2015.1068286
• Languages: English
• ISSNs: 1476-7058

Abstract

Abstract: Objective : Necrotizing enterocolitis has been investigated and debated extensively in recent years; however, there is still no effective treatment. The aim of this study was thus to examine the effects of β-estradiol on intestinal injury in rats. Methods : Twenty-four newborn female rat pups were divided into three groups. In group 1 (sham), hypoxia–re-oxygenation was not performed. In group 2 (saline), the rats were injected with saline after hypoxia–re-oxygenation, and the process was repeated for 5 d. In group 3 (β-estradiol treatment), the rats were subjected to hypoxia–re-oxygenation and then given β-estradiol intraperitoneally once a day for 5 d. After these procedures, the terminal ileum was removed for analysis. Results : Statistically significant differences in histological grades were found between groups 1 and 2 ( p = 0.000), groups 1 and 3 ( p = 0.028), and groups 2 and 3 ( p = 0.021). There were also differences in TNF-α and IL-6 levels between groups 2 and 3 ( p = 0.000 and p = 0.038, respectively) and between groups 1 and 2 ( p = 0.000 and p = 0.000); there was no difference between groups 1 and 3 ( p = 0.574 and p = 0.195, respectively). Electron microscopy examination revealed a decrease in lipid droplets at the apical cytoplasm of the columnar cells in group 2; in group 3, the absorption of the lipids as lipid droplets was similar to that of group 1. Conclusion : In this study, β-estradiol was found to decrease the intensity of intestinal injury significantly by inhibiting TNF-α and IL-6.