Selective adsorption of modified nucleoside cancer biomarkers by hybrid molecularly imprinted adsorbents. Issue 15 (8th July 2016)

• Record Type: Journal Article
• Authors: Iwanowska, Agnieszka; Yusa, Shin‐Ichi; Nowakowska, Maria; Szczubiałka, Krzysztof
• Is Part Of: Journal of separation science. Volume 39:Issue 15(2016)
• Page Start: 3072
• Page End: 3080
• Publication Date: 2016-07-08
• Subjects: Adenine -- Adsorbents -- Cancer biomarkers -- Molecular imprinting -- Thymine; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201600132
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : Modified adenosine nucleosides have been proposed to be potential DNA‐based biomarkers for early diagnosis of tumor and a promising tool for the development of noninvasive prediction systems. However, the low concentration of modified adenosine nucleosides in physiological fluids makes them challenging for both quantitative and qualitative determination. Therefore, materials, which are potentially useful for selective adsorption of nucleobase‐containing compounds, were obtained. To obtain the adsorbents, the silica gel particles were coated layer‐by‐layer with films of the polymers with different combinations of polymers containing thymine groups. Next, the microspheres were irradiated with UV light in the presence of 2'‐deoxyadenosine or 5'‐deoxy‐5'‐(methylthio)adenosine, as template molecules, which resulted in the photodimerization of thymine moieties and molecular imprinting of adsorbed modified adenosine compounds. The selectivity of the adsorption was significantly enhanced by the photoimprinting process. Eventually, the imprinted particles have shown an improved ability to recognize mainly 2'‐deoxyadenosine and 5'‐deoxy‐5'‐(methylthio)adenosine molecules. The best performing adsorbent was obtained using modified natural polysaccharides. The studied materials could serve as promising adsorbents of biomarkers for tumor diagnostics.