Droplet digital PCR and qRT-PCR to detect circulating miR-21 in laryngeal squamous cell carcinoma and pre-malignant laryngeal lesions. (1st September 2016)

• Record Type: Journal Article
• Authors: Wei, Li; Mao, Meiling; Liu, Honggang
• Is Part Of: Acta oto-laryngologica. Volume 136:Number 9(2016)
• Page Start: 923
• Page End: 932
• Publication Date: 2016-09-01
• Subjects: Pre-malignant laryngeal lesion -- laryngeal squamous cell carcinoma -- miRNA-21 -- droplet digital PCR; Otolaryngology -- Periodicals; Ear -- Diseases -- Periodicals; Throat -- Diseases -- Periodicals; Otolaryngology -- Electronic Resources; Otorhinolaryngologic Diseases; 617.8
• DOI: 10.3109/00016489.2016.1165862
• Languages: English
• ISSNs: 0001-6489

Abstract

Abstract: Conclusions : The present study indicates that miR-21 is involved in progression from normal to pre-malignant laryngeal lesions (PLLs) and from PLLs to laryngeal squamous cell carcinoma (LSCC). Furthermore, normalized PCR results for miR-21 might be used to discriminate between normal and ordinary hyperplasia before the emergence of dysplasias and pre-malignant lesions with malignant potential.Objective : To investigate a sensitive marker that contributes to progression from normal tissue to PLLs and from PLLs to LSCC.Methods : In 116 PLLs and LSCC patients and 19 without dysplasia matched sets of tissue and plasma samples from Beijing Tongren Hospital, miR-21 was analysed by droplet digital PCR and quantitative real-time polymerase chain reaction based on paraffin-embedded tumour tissue and plasma.Results : Compared with controls, miR-21 levels in tissue and plasma were significantly higher for both PLL and LSCC groups (for both groups vs controls: p < 0.0001 for tissue; p < 0.05 for plasma). Areas under receiver-operating curves (AUC) for tissue miR-21 were PLL group vs controls: 0.9026 and LSCC group vs controls: 0.8681. For plasma miR-21, AUCs were PLL group vs controls: 0.7355 and LSCC group vs control: 0.6723.