Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea. (2nd June 2016)

• Record Type: Journal Article
• Authors: Adachi, Hiroaki; Ishihama, Nobuaki; Nakano, Takaaki; Yoshioka, Miki; Yoshioka, Hirofumi
• Is Part Of: Plant signaling & behavior. Volume 11:Number 6(2016)
• Publication Date: 2016-06-02
• Subjects: Botrytis cinerea -- cell death -- HR -- MAPK -- MAPKK -- necrotroph -- Nicotiana benthamiana -- RBOH -- WRKY; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2016.1183085
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: MEK2-SIPK/WIPK cascade, a Nicotiana benthamiana mitogen-activated protein kinase (MAPK) cascade, is an essential signaling pathway for plant immunity and involved in hypersensitive response (HR) accompanied by cell death. WRKY transcription factors as substrates of SIPK and WIPK have been isolated and implicated in HR cell death. Here, we show virus-induced gene silencing of WRKY genes compromised constitutively active MEK2-triggered cell death in N. benthamiana leaves. In general, HR cell death enhances susceptibility to necrotrophic pathogens such as Botrytis cinerea . However, the WRKY gene silencing elevated susceptibility to B. cinerea . These findings suggest that downstream WRKYs of MEK2-SIPK/WIPK cascade are required for cell death-dependent and -independent immunities in N. benthamiana .