Forkhead box O member FOXO1 regulates the majority of follicle-stimulating hormone responsive genes in ovarian granulosa cells. (15th October 2016)

• Record Type: Journal Article
• Authors: Herndon, Maria K.; Law, Nathan C.; Donaubauer, Elyse M.; Kyriss, Brandon; Hunzicker-Dunn, Mary
• Is Part Of: Molecular and cellular endocrinology. Volume 434(2016)
• Page Start: 116
• Page End: 126
• Publication Date: 2016-10-15
• Subjects: FOXO1 -- Granulosa cells -- Follicle-stimulating hormone -- RNA deep sequencing; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2016.06.020
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: FSH promotes maturation of ovarian follicles. One pathway activated by FSH in granulosa cells (GCs) is phosphatidylinositol-3 kinase/AKT. The AKT target FOXO1 is reported to function primarily as a repressor of FSH genes, including Ccnd2 and Inha . Based on its broad functions in other tissues, we hypothesized that FOXO1 may regulate many more GC genes. We transduced GCs with empty adenovirus or constitutively active FOXO1 followed by treatment with FSH for 24 h, and conducted RNA deep sequencing. Results show that FSH regulates 3772 genes ≥2.0-fold; 60% of these genes are activated or repressed by FOXO1. Pathway Studio Analysis revealed enrichment of genes repressed by FOXO1 in metabolism, signaling, transport, development, and activated by FOXO1 in signaling, cytoskeletal functions, and apoptosis. Gene regulation was verified by q-PCR (eight genes) and ChIP analysis (two genes). We conclude that FOXO1 regulates the majority of FSH target genes in GCs. Highlights: RNA deep sequencing detected 13, 461 genes in primary rat ovarian granulosa cells. RNA deep sequencing showed that FSH regulates 3772 genes ≥2.0-fold. Sixty % of the genes regulated by FSH are activated or repressed by FOXO1. Pathway Studio Analysis revealed enrichment of genes repressed by FOXO1 in metabolism, signaling, transport, and development. Pathway Studio Analysis revealed enrichment of genes activated by FOXO1 in signaling, cytoskeletal functions, and apoptosis.