Antioxidant, antilipidemic and antidiabetic effects of ficusin with their effects on GLUT4 translocation and PPARγ expression in type 2 diabetic rats. (25th August 2016)

• Record Type: Journal Article
• Authors: Irudayaraj, Santiagu Stephen; Stalin, Antony; Sunil, Christudas; Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah; Ignacimuthu, Savarimuthu
• Is Part Of: Chemico-biological interactions. Volume 256(2016)
• Page Start: 85
• Page End: 93
• Publication Date: 2016-08-25
• Subjects: Ficus carica -- Ficusin -- GLUT4 -- PPARγ -- High fat diet -- Streptozotocin; HFD-STZ high-fat diet fed – streptozotocin -- OGTT oral glucose tolerance test -- ITT insulin tolerance test -- SOD superoxide dismutase -- CAT catalase -- GPx glutathione peroxidase -- TC total cholesterol -- TG triglycerides -- FFA free fatty acids -- PPARγ peroxisome proliferator-activated receptor γ -- GLUT4 glucose transporter protein 4 -- LDL low-density lipoprotein -- VLDL very low-density Lipoprotein -- HDL high-density lipoprotein -- CHD coronary heart disease -- WHO world health organization -- TLC thin layer chromatography -- UV ultra violet -- 1H NMR 1H nuclear magnetic resonance -- 13C NMR 13C nuclear magnetic resonance -- IR infra red -- ALT serum glutamate pyruvate transaminases -- AST serum glutamate oxaloacetate transaminases -- ALP alkaline phosphatase -- ANOVA analysis of variance -- SEM standard error mean -- RT-PCR reverse transcriptase-polymerase chain reaction; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2016.06.023
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: In this study, the antioxidant, antilipidemic and antidiabetic effects of ficusin isolated from Ficus carica leaves and their effects on GLUT4 translocation and PPARγ expression were evaluated in HFD-STZ induced type 2 diabetic rats. Ficusin (20 and 40 mg/kg b. wt.) lowered the levels of fasting blood glucose, plasma insulin and body weight gain, in HFD-STZ induced diabetic rats. Ficusin also significantly lowered the serum antioxidant enzymes (SOD, CAT and GPx) and lipids (TC, TG and FFA) levels to near normal. Ficusin significantly enhanced the PPARγ expression and improved the translocation and activation of GLUT4 in the adipose tissue. Molecular docking analysis exhibited promising interactions of GLUT4 and PPARγ into their active sites. This study suggests that ficusin improved the insulin sensitivity on adipose tissue and it can be used for the treatment of obesity related type 2 diabetes mellitus. Highlights: Ficusin was isolated from Ficus carica leaves. Ficusin restored the body weight, blood glucose, plasma insulin and lipid levels to normal. Ficusin increased the PPARγ expression in adipose tissue. Ficusin enhanced GLUT4 translocation and activation. Ficusin docked with PPARγ and GLUT4.