Immobilization of Cellulase on Magnetic Nanocarriers. Issue 3 (29th April 2016)

• Record Type: Journal Article
• Authors: Roth, Hans‐Christian; Schwaminger, Sebastian P.; Peng, Fei; Berensmeier, Sonja
• Is Part Of: ChemistryOpen. Volume 5:Issue 3(2016:Jun.)
• Page Start: 183
• Page End: 187
• Publication Date: 2016-04-29
• Subjects: enzyme catalysis -- immobilization -- magnetic separation -- nano-biotechnology -- nanomaterials; Chemistry -- Periodicals; 540; 540.5
• DOI: 10.1002/open.201600028
• Languages: English
• ISSNs: 2191-1363

Abstract

Abstract: The constant increase in the number of sustainable products on the global markets demands new biotechnological processing strategies such as the purification and recovery of biocatalysts. Superparamagnetic iron oxide nanoparticles exhibit excellent recovery properties as carrier materials in enzyme catalysis. We present the simple and fast electrostatic assembly of cellulase (CEL) and low‐priced silica‐coated magnetic nanoparticles, which demonstrates stable enzyme bonding and excellent colloidal stability. The high CEL loading (0.43 g g −1 ), without leaching of biocatalyst and high recovery yields (75 %), could be sustained over ten magnetic recycling steps. The highlight of this study is the preservation of a high enzymatic activity and, therefore, the outstandingly high lifecycle stability. Abstract : Small particles, great potential : Magnetic nanoscale carrier materials can reduce negative mass‐transfer effects and thus facilitate high enzymatic activity in the hydrolysis and degradation of highly molecular cellulose to single reducing sugars, such as glucose. We immobilized cellulase on bare and silica‐coated magnetite nanoparticles and were able to achieve high loadings and no loss in activity over ten process cycles.