A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single‐Cell Metabolism in Droplet‐Based Microfluidics. (1st June 2016)

• Record Type: Journal Article
• Authors: Del Ben, Fabio; Turetta, Matteo; Celetti, Giorgia; Piruska, Aigars; Bulfoni, Michela; Cesselli, Daniela; Huck, Wilhelm T. S.; Scoles, Giacinto
• Is Part Of: Angewandte Chemie. Volume 128:Number 30(2016)
• Page Start: 8723
• Page End: 8726
• Publication Date: 2016-06-01
• Subjects: Krebs -- Mikrofluidik -- Pikotröpfchen -- Stoffwechsel -- Zirkulierende Tumorzellen; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201602328
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: The number of circulating tumor cells (CTCs) in blood is strongly correlated with the progress of metastatic cancer. Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label‐free method is presented exploiting the abnormal metabolic behavior of cancer cells. A single‐cell analysis technique is used to measure the secretion of acid from individual living tumor cells compartmentalized in microfluidically prepared, monodisperse, picoliter (pL) droplets. As few as 10 tumor cells can be detected in a background of 200 000 white blood cells and proof‐of‐concept data is shown on the detection of CTCs in the blood of metastatic patients.