Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization. Issue 1 (September 2016)

• Record Type: Journal Article
• Authors: Blanco-Lobo, P.; González-Galán, V.; García-Quintanilla, M.; Valencia, R.; Cazalla, A.; Martín, C.; Alonso, I.; Pérez-Romero, P.; Cisneros, J.M.; Aznar, J.; McConnell, M.J.
• Is Part Of: Journal of hospital infection. Volume 94:Issue 1(2016)
• Page Start: 68
• Page End: 71
• Publication Date: 2016-09
• Subjects: Acinetobacter baumannii -- Active surveillance -- Real-time PCR -- Colonization; Cross infection -- Periodicals; Cross infection -- Prevention -- Periodicals; Nosocomial infections -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Cross Infection -- Periodicals; Cross Infection -- prevention & control -- Periodicals; Infection Control -- Periodicals; Electronic journals; 614.44
• DOI: 10.1016/j.jhin.2016.04.008
• Languages: English
• ISSNs: 0195-6701

Abstract

Summary: Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind study demonstrated that a real-time PCR assay had high sensitivity (100%) and specificity (91.2%) compared with conventional culture for detecting A. baumannii in 397 active surveillance samples, and provided results within 3 h. Receiver-operator curve analyses demonstrated that the technique has diagnostic accuracy of 97.7% (95% confidence interval 96.0–99.3%). This method could facilitate the rapid implementation of infection control measures for preventing the transmission of A. baumannii .