Chemically Engineered Nanoparticle–Protein Interface for Real‐Time Cellular Oxidative Stress Monitoring. Issue 28 (13th June 2016)

• Record Type: Journal Article
• Authors: Jiang, Ying; Wang, Ming; Hardie, Joseph; Tonga, Gulen Yesilbag; Ray, Moumita; Xu, Qiaobing; Rotello, Vincent M.
• Is Part Of: Small. Volume 12:Issue 28(2016)
• Page Start: 3775
• Page End: 3779
• Publication Date: 2016-06-13
• Subjects: cellular oxidative stress -- chemically modified protein -- gold nanoparticles -- green fluorescent proteins -- hydrogen peroxide; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201600986
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : A co‐engineered nanoparticle/protein peroxide detector is created . This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP‐Gal) that reacts covalently with a boronate‐modified green fluorescent protein (PB‐GFP). Boronate acid‐saccharide complexation between PB‐GFP and AuNP‐Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time.