Antibiotic and metal resistance of cultivable bacteria in the Antarctic sea urchin. Issue 4 (5th May 2016)

• Record Type: Journal Article
• Authors: González-Aravena, M.; Urtubia, R.; Del Campo, K.; Lavín, P.; Wong, C.M.V.L.; Cárdenas, C.A.; González-Rocha, G.
• Is Part Of: Antarctic science. Volume 28:Issue 4(2016)
• Page Start: 261
• Page End: 268
• Publication Date: 2016-05-05
• Subjects: Antarctica, -- coelomic fluid, -- multi-resistance, -- Sterechinus neumayeri; Science -- Antarctica -- Periodicals; 509.989
• DOI: 10.1017/S0954102016000109
• Languages: English
• ISSNs: 0954-1020

Abstract

Abstract: In this paper we report the first characterization of cultivable bacteria obtained from the Antarctic sea urchin Sterechinus neumayeri . The coelomic fluid was obtained from a pool of sea urchins which was plated onto different media to isolate the bacteria. A total of 42 isolates of psychrotrophic and aerobic γ-Proteobacteria (59.5%), Flavobacteria (33.3%) and Actinomycetes (7.2%) were isolated and sequenced. These bacteria were exposed to heavy metals and antibiotics, where 38 strains were analysed by the minimal inhibitory concentration method. Antibiotic resistance was detected in 44% of cultivable strains, and a further 13% presented co-resistance to antibiotics and heavy metals. The genera of bacteria that showed an increased resistance and co-resistance to metals and antibiotics were Flavobacterium, Psychrobacter and Pseudomonas . Additionally, 30.9% of isolated bacterial strains contained plasmids, which are probably related to resistance and co-resistance to metals. These results indicate that sea urchin-associated bacteria could be reservoirs for antibiotic resistance genes.