Introgression of clubroot resistance into an elite pak choi inbred line through marker‐assisted introgression breeding. Issue 4 (24th May 2016)

• Record Type: Journal Article
• Authors: Chen, Longzheng; Zhang, Xiaoqing; Xu, Hai; Song, Bo; Fan, Xiaoxue
• Editors: Snowdon, R.
• Is Part Of: Plant breeding. Volume 135:Issue 4(2016)
• Page Start: 471
• Page End: 475
• Publication Date: 2016-05-24
• Subjects: pak choi -- Chinese cabbage -- clubroot resistance -- introgression hybrid -- molecular marker; Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12379
• Languages: English
• ISSNs: 0179-9541

Abstract

Abstract: Clubroot is a soilborne disease that severely infects cruciferous species. Pak choi ( Brassica rapa subsp. chinensis ) is an economically important cruciferous crop cultivated throughout the world. However, no clubroot‐resistant germplasms have been identified in pak choi to date. To improve disease resistance, we used marker‐assisted selection (MAS) to introgress the clubroot resistance (CR) trait from the 'CCR13685' Chinese cabbage ( B . rapa subsp . pekinensis ) inbred line into an elite pak choi inbred line, 'GHQ11021'. Genetic analysis of F2 and BC1 progeny showed that CR of 'CCR13685' was controlled by a single dominant gene. We designed nine candidate sequence‐characterized amplified region markers, K‐1 to K‐9, based on two molecular markers linked to the CR gene. We found that K‐3 co‐segregated with CR and an inoculation test confirmed that K‐3 could be used for MAS. Two introgression lines, BC3 ‐1‐4 and BC3 ‐2‐18, were developed using K‐3 for foreground selection. These lines displayed the same phenotypic properties as 'GHQ11021', but were highly resistant to clubroot, indicating that the CR gene of 'CCR13685' had been successfully introduced into pak choi.