Mapping of male sterility gene ms10 in chilli pepper (Capsicum annuum L.). Issue 4 (27th June 2016)

• Record Type: Journal Article
• Authors: Aulakh, Parjeet Singh; Dhaliwal, Major S.; Jindal, Salesh Kumar; Schafleitner, Roland; Singh, Kuldeep
• Editors: Havey, M.
• Is Part Of: Plant breeding. Volume 135:Issue 4(2016)
• Page Start: 531
• Page End: 535
• Publication Date: 2016-06-27
• Subjects: chilli pepper -- gene mapping -- molecular markers -- ms10 -- nuclear male sterility; Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12389
• Languages: English
• ISSNs: 0179-9541

Abstract

Abstract: Most of the hybrid seed in chilli are produced manually, but the use of male sterility (MS) can reduce the cost of hybrid seed production. MS‐12, a nuclear male‐sterile (NMS) line developed at Punjab Agricultural University, Ludhiana (India), has been utilized to develop commercial F1 hybrids. A recessive gene, designated as ms10, governs MS in MS‐12. Due to recessive gene control, development of new NMS lines incorporating ms10 gene is tedious and time‐consuming. We identified SSR markers AVRDC‐PP12 and AVRDC_MD997* linked to the ms10 gene. A total of 558 primer pairs were screened following bulked segregant analysis (BSA). Linkage analysis in 210 F2 plants indicated that the two SSR markers were linked to the ms10 gene and the marker AVRDC‐PP12 was closest to the gene at 7.2 cM distance. The marker was mapped to chromosome 1 at genome position 175 694 513 to 175 694 644. Until more closely linked markers are developed, the marker AVRDC‐PP12 would facilitate transfer of ms10 gene through marker‐assisted selection (MAS). Fine mapping would lead to cloning of the ms10 gene.