Novel Reaction‐Based Fluorescence Probes for the Detection of Hydrogen Sulfide in Living Cells. Issue 11 (18th July 2016)

• Record Type: Journal Article
• Authors: Zhao, Zhensheng; Cao, Lixia; Zhang, Tao; Hu, Rui; Wang, Shuangqing; Li, Shayu; Li, Yi; Yang, Guoqiang
• Is Part Of: ChemistrySelect. Volume 1:Issue 11(2016)
• Page Start: 2581
• Page End: 2585
• Publication Date: 2016-07-18
• Subjects: Fluorescence Probes -- Hydrogen Sulfide -- tetrazine group -- BODIPY -- 3T3 fibroblast cells; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201600382
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Hydrogen Sulfide (H2 S), a kind of important gas signal molecules, serves as an important medium in physiological and pathological studies. It is also a hot spot in the research of chemistry and biology. We now report a new type of reaction‐based fluorescent probe for selective detecting H2 S in living cells that adopts H2 S reaction with tetrazine group (Tz) to dihydio‐tetrazine. The fluorescence probes, BODIPY−Tz‐I and BODIPY−Tz‐II (BODIPY: abbreviation for boron‐dipyrromethene), respond to H2 S by a turn‐on fluorescence signal enhancement and show high selectivity of H2 S over other biologically species. BODIPY−Tz‐I and BODIPY−Tz‐II can be used to detect H2 S in both buffer solution and cells in vivo, which provide a novel and effective approach for H2 S detection in biological systems. Abstract : Hydrogen Sulfide (H2 S), a kind of important gas signal molecules, serves as an important medium in physiological and pathological studies. It is also a hot spot in the research of chemistry and biology. We now report a new type of reaction‐based fluorescent probe for selective detecting H2 S in living cells that adopts H2 S reaction with tetrazine group (Tz) to dihydio‐tetrazine. The fluorescence probes, BODIPY−Tz‐I and BODIPY−Tz‐II, respond to H2 S by a turn‐on fluorescence signal enhancement and show high selectivity of H2 S over other biologically species. BODIPY−Tz‐I and BODIPY−Tz‐II can be used to detect H2 S in both buffer solution and in vivo cells, which provide a novel and effective approach for H2 S detection in biological systems.