Fluorescence alarming ON–OFF–ON switch derived from biocompatible carbon nanoparticle–hemoglobin–H2O2 interaction. Issue 74 (26th July 2016)

• Record Type: Journal Article
• Authors: Singh Rana, Prem Jyoti; Singh, Pallavi; Kar, Prasenjit
• Is Part Of: RSC advances. Volume 6:Issue 74(2016)
• Page Start: 70660
• Page End: 70668
• Publication Date: 2016-07-26
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra14308c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The present study comprises a deeper comprehension on the applicability of environmentally benign water soluble fluorescent Carbon Nanoparticles (CNs) synthesised from jamun ( Syzygium cumini ). Abstract : The present study comprises a deeper comprehension on the applicability of environmentally benign water soluble fluorescent Carbon Nanoparticles (CNs) synthesised from jamun ( Syzygium cumini ). We have synthesised two types of CNs i.e., surface non-passivated and surface passivated. The morphological investigation of either type of CN shows a narrow particle size distribution with an average size of 40 nm and 42 nm respectively. These CNs show potential candidacy as cell imaging agents due to cell viability up to a high concentration of 30 μg mL −1 as revealed by cytotoxicity analysis. Furthermore, we employed hemoglobin as a target to explore the biosensing ability of CNs based on a turn ON–OFF mechanism. Moreover, we have made a reversible fluorescent turn ON–OFF–ON switch taking the CNs–Hb composite system in conjugation with a large excess H2 O2 . This unbelievable fluorescence enhancement of the CNs–Hb composite system on exposure to excessive H2 O2 is due to a decrement in particle size that conversely increases the surface area to volume ( S / V ) ratio which in turn intensifies the PL spectra. Using the above intriguing changes, a nanoparticle based implication logic operation (IMP) was created with Hb and H2 O2 as inputs.