Rare sugar production by coupling of NADH oxidase and l-arabinitol dehydrogenase. Issue 71 (14th July 2016)

• Record Type: Journal Article
• Authors: Gao, Hui; Kim, Tae-Su; Mardina, Primata; Zhou, Pengji; Wen, Fei; Lee, Jung-Kul
• Is Part Of: RSC advances. Volume 6:Issue 71(2016)
• Page Start: 66609
• Page End: 66616
• Publication Date: 2016-07-14
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra11614k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : An efficient biocatalytic cell-free system containingl -arabinitol dehydrogenase (LAD) forl -arabinitol oxidation and NADH oxidase (Nox) for cofactor regeneration was successfully constructed and used forl -rare sugar production. Abstract : An efficient biocatalytic cell-free system containingl -arabinitol dehydrogenase (LAD) forl -arabinitol oxidation and NADH oxidase (Nox) for cofactor regeneration was successfully constructed and used forl -rare sugar production. The recombinant LAD (HjLAD) from Hypocrea jecorina suffered from NADH inhibition. Applying Nox to the cell-free HjLAD system drove the thermodynamically unfavorable equilibrium froml -arabinitol tol -xylulose, along with the regeneration of the oxidized cofactor NAD + . Response surface methodology was used to optimize the conditions forl -xylulose production. The optimal conditions for biocatalytic production ofl -xylulose were found to be 4.9 U mL −1 HjLAD, 8.2 mM NAD + at pH 8.0, and 30.9 °C; a high conversion rate of 78.4% was achieved under these conditions. We demonstrate a cell-free enzyme coupling system enabling efficient cofactor recycling and providing high yields ofl -xylulose. The results indicate that this coupling system provides a new biocatalytic method forl -rare sugar production.