A carbon dot–hemoglobin complex-based biosensor for cholesterol detection. Issue 15 (17th May 2016)

• Record Type: Journal Article
• Authors: Bui, Trang Thi; Park, Soo-Young
• Is Part Of: Green chemistry. Volume 18:Issue 15(2016)
• Page Start: 4245
• Page End: 4253
• Publication Date: 2016-05-17
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/c6gc00507a
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. Abstract : In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π–π interactions between CD and Hb in the CD/Hb complex. CD is released from the CD/Hb complex because hydrophobic interactions between Hb and cholesterol are more favorable than π–π interactions between CD and Hb. The CD/Hb complex enabled selective detection of cholesterol within a linear range from 0 to 800 μM, with a limit of detection of 56 μM and a response time of ≤5 minutes in human blood plasma. Compared with other cholesterol sensors, a CD/Hb complex-based biosensor is simple, highly sensitive, selective, rapid, eco-friendly, and cost-effective for cholesterol detection in both biological and environmental samples.