Bovine lactoferrin allergenicity as studied in murine model of allergy. Issue 5 (2nd September 2016)

• Record Type: Journal Article
• Authors: Negaoui, Hanane; El Mecherfi, Kamel Eddine; Tadjer, Sid Ahmed; Grar, Hadria; Kheroua, Omar; Saidi, Djamel
• Is Part Of: Food and agricultural immunology. Volume 27:Issue 5(2016)
• Page Start: 711
• Page End: 723
• Publication Date: 2016-09-02
• Subjects: Lactoferrin -- allergen -- Balb/c -- gut inflammation -- anaphylactic reaction; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2016.1160365
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: In our study, we tried to investigate the allergenic properties of bovine lactoferrin (bLf) at different doses. The lactoferrin (LF) allergenicity was explored using murine model of allergy through measuring anti-LF IgG, IgG1 and IgE antibodies (Abs) responses and by in vivo anaphylactic reactions in LF sensitized mice. A histological examination of intestinal lamina propria was performed to assess gut inflammation. Four groups of mice (Balb/c) were sensitized intraperitoneally to bLf using different doses (1%, 2%, 5% and 10%). Histological study of intestinal lamina propria revealed a marked decrease in villus length in 5% and 10% sensitized mice groups. Also, high levels of anti-bLf IgG and IgE were observed. Concerning the in vivo reactions, all groups developed clinical symptoms of anaphylactic reactions at different stages. Our findings show that LF-sensitized mice at 5% and 10% developed important clinical symptoms after intraperitoneal challenge with LF. GRAPHICAL ABSTRACT: