Identification and characterization of Dichelobacter nodosus serogroup H from ovine footrot in India. (August 2016)

• Record Type: Journal Article
• Authors: Vinod Kumar, N.; Sreenivasulu, D.; Karthik, A.
• Is Part Of: Anaerobe. Volume 40(2016)
• Page Start: 100
• Page End: 102
• Publication Date: 2016-08
• Subjects: Andhra Pradesh -- Dichelobacter nodosus -- fimA gene -- Footrot -- Serogroup H -- India; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2016.05.013
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: A total of 56 foot swabs were collected from inter digital spaces of sheep with footrot lesions were screened for 16 rRNA of Dichelobacter nodosus by PCR. Out of the 56 samples, 38(67.85%) were found to be positive. All the positive samples were subjected to multiplex PCR targeting fimA gene for identification of serogroups of D. nodosus . Serogroup H was found along with serogroup B in 12 (55.26%) samples and with serogroup I in 8 (22.2%) samples. The serogroup H was identified for the first time from the Indian subcontinent. The phylogenetic analysis of the present sequence with the available serogroup H sequences of GenBank revealed to be in close association with the serotype H1. Highlights: The article deals with the surveillance of Dichelobacter nodosus serogroups in the region. Present paper deals with the report of serogroup H for the first time in the region. Availability of only five serogroup H sequences in GenBank and scarcity of literature indicates the significance of present finding. The study also explores the need to identify and include the serotypes within the serogroups of D. nodosus in vaccine preparation for control of footrot.