Antioxidation and anti-ageing activities of different stereoisomeric astaxanthin in vitro and in vivo. (August 2016)

• Record Type: Journal Article
• Authors: Liu, Xiaojuan; Luo, Qingxin; Rakariyatham, Kanyasiri; Cao, Yong; Goulette, Timothy; Liu, Xin; Xiao, Hang
• Is Part Of: Journal of functional foods. Volume 25(2016)
• Page Start: 50
• Page End: 61
• Publication Date: 2016-08
• Subjects: Astaxanthin -- Stereoisomeric -- Antioxidation -- Anti-ageing -- Cellular antioxidant activity -- Caenorhabditis elegans; Astaxanthin (PubChem CID: 2246) -- DPPH (PubChem CID: 2735032) -- ABTS (PubChem CID: 16240279) -- Trolox (PubChem CID: 40634) -- AAPH (PubChem CID: 1969) -- TBHP (PubChem CID: 6410) -- DCFH-DA (PubChem CID: 104913) -- Fluorescein (PubChem CID: 16850) -- EDTA (PubChem CID: 6049) -- MTT (PubChem CID: 64965); AST astaxanthin -- S 3S, 3'S -- R 3R, 3'R -- M a statistical mixture -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- ABTS 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt -- ORAC oxygen radical absorbance capacity -- C. elegans Caenorhabditis elegans -- CAA cellular antioxidant activity -- ROS reactive oxygen species -- SOD superoxide dismutase -- CAT catalase -- trolox 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid -- AAPH 2, 2′-Azobis (2-amidinopropane) dihydrochloride -- DMEM Dulbecco's modification of eagle's medium -- FBS foetal bovine serum -- EDTA ethylenediaminetetraacetic acid -- TBHP 1, 1-Dimethylethyl hydroperoxide, 2-Hydroperoxy-2-methylpropane -- NGM nematode growth medium -- DCFH-DA 2, 7-dichlorodihydrofluorescein diacetate; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.05.009
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: There were significant differences among three stereoisomeric AST both in vitro and in vivo . S AST exhibited the strongest antioxidation and anti-ageing activities. Anti-ageing activities of three stereoisomeric AST do not cause adverse effects on the physiological functions of C. elegans . Abstract: Astaxanthin (AST) is a potent antioxidant with different stereoisomers in nature. However, there have been no reports about the functional activities of different stereoisomers so far. In this study, the antioxidant and anti-ageing activities of 3S, 3'S ( S ), 3R, 3'R ( R ), and mixture ( M ) AST were evaluated in chemical-based assays, cellular antioxidant assays (CAA), and an organism-based model. S AST exhibited the strongest activity in chemical assays and CAA. The median lifespan of Caenorhabditis elegans fed with S, R and M AST was found to increase by 27.61%, 25.25% and 22.69%, respectively. The three stereoisomeric AST did not cause adverse effects on the physiological functions of C . elegans . A decreasing trend of intracellular ROS accumulation caused by AST treatment was accompanied by the prolonged lifespan. Our results provided mechanistic information on the antioxidation and anti-ageing activities of different stereoisomeric AST, which is useful for rational utilization of AST in functional food.